Simultaneous measurements of anti-exudation and anti-granulation activities of antiinflammatory steroids

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  • 抗炎症ステロイドの抗滲出作用および抗肉芽作用の同時測定について
  • コウエンショウ ステロイド ノ コウシンシュツサヨウ オヨビ コウニクガ サヨウ ノ ドウジ ソクテイ ニ ツイテ

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The Selye's granuloma pouch method of anti-inflammatory assay was improved by injecting nitrogen gas instead of air for making a subcutaneous pouch in the back of the rat. Croton oil inflamed exdation in the nitrogen-pouch was much greater in the exudate volume and apparently smaller in the variance of individual volume when compared with the croton oil exudation in the air-pouch. Accordingly, the precision of antiexudation assay was improved. It was further demonstrated that the previously reported formalin-filterpaper-pellet method could be additionally applied in the rats bearing the nitrogen-pouch exudation, so that the simultaneous measurements of anti-granulation and anti-exudation activities of the test compound were available. Structure-activity relations of various pregn-4-ene derivatives were reinvestigated by the above method, and it was concluded that anti-exudation activities of steroidal compounds did not necessarily corelate with their anti-granulation activities: antiexudation activity of progesterone was increased by 17α-hydroxylation but decreased by 21-hydroxylation, and the coexistence of 11β-OH and 21-OH was needed for the manifestation of granuloma inhibition and of adrenal and thymus involution.

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