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- OKA Motoo
- Department of Pharmacology, Tokushima University School of Medicine
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- MORITA Kyoji
- Department of Pharmacology, Tokushima University School of Medicine
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- YOSHIZUMI Masanori
- Department of Pharmacology, Tokushima University School of Medicine
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- HOUCHI Hitoshi
- Department of Pharmacology, Tokushima University School of Medicine
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- ISHIMURA Yasuko
- Department of Pharmacology, Tokushima University School of Medicine
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- MASUDA Yutaka
- Department of Pharmacology, Tokushima University School of Medicine
Bibliographic Information
- Other Title
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- シグナル伝達と細胞機能調節 副腎クロマフィン細胞からのカテコールアミン分泌と薬物作用
- フクジン クロマフィン サイボウ カラ ノ カテコールアミン ブンピ ト ヤク
- シグナル伝達と細胞機能調節
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Abstract
The effect of palytoxin (PTX) on catecholamine (CA) secretion from cultured bovine adrenal chromaffin cells was examined. PTX (> 10-10 M) induced CA secretion concentration-dependently. About 40 ?? 50% of the total cellular CA was secreted during a 20 min incubation with 3 × 10-8 M PTX. PTX caused increases in [22Na]+ and [95Ca]2+-influxes into the cells, which were not affected by TTX. PTX-induced CA secretion and [22Na]+ and [45Ca]2+-influxes were significantly inhibited by quinidine and aprindine, antiarrythmic drugs. Ca2+-channel blockers such as nifedipine, verapamil, Co2+, and Cd2+ inhibited both CA secretion and [45Ca]2+-influx induced by PTX. These results indicated that PTXinduced CA secretion was mediated by activation of Na+-dependent, TTX-insensitive voltage-dependent Ca2+-channels. PTX-induced [22Na]+-influx was inhibited by amiloride, an inhibitor of the Na+-H+ exchange system, suggesting that the Na+-H+ exchange mechanism might be involved in PTX-induced [22Na]+-influx into the cells. The effects of flavonoids on CA secretion from permeabilized adrenal chromaffin cells were examined. CA secretion from the cells in response to a direct Ca2+ challenge was inhibited by quercetin (> 10-5 M) and apigenin (> 10-5 M). These flavonoids also inhibited phorbol ester TPA-induced CA secretion. Therefore, the inhibitory effects of flavonoids on CA secretion were thought to be attributed to their inhibitory effects on PKC.
Journal
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- Folia Pharmacologica Japonica
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Folia Pharmacologica Japonica 98 (3), 209-214, 1991
The Japanese Pharmacological Society
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Keywords
Details 詳細情報について
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- CRID
- 1390001204271561472
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- NII Article ID
- 130000758869
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- NII Book ID
- AN00198335
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- ISSN
- 13478397
- 00155691
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- NDL BIB ID
- 3736960
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- PubMed
- 1683853
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed