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- KIMURA Fumio
- Department of Pharmacology, Nippon Medical School
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- SHIMOZAWA Junko
- Department of Pharmacology, Nippon Medical School
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- SAITO Fumiko
- Department of Pharmacology, Nippon Medical School
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- MORIKUBO Mari
- Department of Pharmacology, Nippon Medical School
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- TAKEUCHI Setsuya
- Department of Pharmacology, Nippon Medical School
Bibliographic Information
- Other Title
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- インスリン分泌研究のための新しいすいかん流法
- Insulin ブンピ ケンキュウ ノ タメ ノ アタラシイ スイ カンリュウ
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Abstract
A new perfusion technique was applied to the rat pancreas to elucidate neural mechanisms related to insulin secretion. The pancreas and a part of the gastroduodenal tract were perfused in situ with an artificial perfusion fluid consisting of 5% of rat red blood cells, 4% bovine serum albumin and 0.1 % of glucose and Krebs-Ringer bicarbonate buffer. Stimulation (5-10V, 5 msec, 50 Hz for 5 min) to the right or bilateral cervical vagal nerves produced a significant increase in insulin activity in the perfusate. Acetylcholine (5-10 μg/0.1 ml) added to the perfusion fluid produced a dose-dependent increase in insulin activity in the perfusate, and the responses disappeared after pretreatment with atropine (100 μg/ml in perfusion fluid). After adding noradrenaline or isoproterenol (each 0.1 μg/0.1 ml) to the perfusion fluid, appreciable changes were not apparent.
Journal
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- Folia Pharmacologica Japonica
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Folia Pharmacologica Japonica 77 (2), 213-220, 1981
The Japanese Pharmacological Society
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Keywords
Details 詳細情報について
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- CRID
- 1390001204273316096
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- NII Article ID
- 130000759335
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- NII Book ID
- AN00198335
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- COI
- 1:CAS:528:DyaL3MXhtlKksbo%3D
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- ISSN
- 13478397
- 00155691
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- NDL BIB ID
- 2344940
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- PubMed
- 7016692
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed