Neurotoxicity of .BETA.-amyloid.
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- KANEKO Isao
- Neuroscience and Immunology Research: Laboratories, SANKYO CO.,LTD.
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- KUBO Takekazu
- Neuroscience and Immunology Research: Laboratories, SANKYO CO.,LTD.
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- MORIMOTO Kiyoshi
- Neuroscience and Immunology Research: Laboratories, SANKYO CO.,LTD.
Bibliographic Information
- Other Title
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- βアミロイドによる神経細胞毒性
- ベータ アミロイド ニ ヨル シンケイ サイボウ ドクセイ
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Abstract
Recent observations on the neurotoxicity of β-amyloid have been reviewed and possible roles of racemization of β-amyloid are discussed. β1-40, β25-35 and D-Ser26β25-35 (all HCl salt forms), but not commercially available β1-40 (TFA salt form), take the β-structure within few hours in PBS, form fibrils, exert toxic effects on hippocampal cultured neurons and suppresses MTT reduction activity of non-neuronal HeLa cells without cytotoxicity. D-Ser26β1-40 is soluble and non-toxic in vitro but is converted by brain proteinases to D-Ser26β25-35, a potent toxic and proteinase-resistant fragment. The co-injection of β1-40, D-Ser26β25-35 or D-Ser26β1-40 with ibotenic acid, but not β-amyloid alone or ibotenic acid alone, into rat brains produce drastic neuronal loss in the hippocampal CA1 area. The in vivo degeneration activity of β-amyloids is well correlated with their having β-structure and activity to suppress the MTT reduction activity. A specific antibody against D-Ser26β25-35 strongly reacts with hippocampal degenerated-CA1 neurons in AD but not control brains. These results suggest that D-Ser26β25-35 and related peptides possibly generated from insoluble β1-40 due to aging exert toxic effects on the hippocampal CA1 pyramidal neurons by enhancing the susceptibility to excitatory amino acids.
Journal
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- Folia Pharmacologica Japonica
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Folia Pharmacologica Japonica 115 (2), 67-77, 2000
The Japanese Pharmacological Society
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Details 詳細情報について
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- CRID
- 1390001204273328640
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- NII Article ID
- 10005705483
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- NII Book ID
- AN00198335
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- COI
- 1:CAS:528:DC%2BD3cXpsVChtA%3D%3D
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- ISSN
- 13478397
- 00155691
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- NDL BIB ID
- 4974737
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- PubMed
- 10876793
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed