Introduction of the conventional method of DNA transfection by adenovirus vector.

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  • OKIYONEDA Tsukasa
    Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Kumamoto University
  • KAI Hirofumi
    Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Kumamoto University

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Other Title
  • アデノウイルスを用いた遺伝子導入法
  • 実験技術 アデノウイルスを用いた遺伝子導入法
  • ジッケン ギジュツ アデノウイルス オ モチイタ イデンシ ドウニュウホウ

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DNA transfection techniques are necessary to perform the high throughput screening (HTS) for drug discovery. Among many transfection techniques, we will recommend the adenovirus vector for HTS as a particularly useful method. The adenovirus vector has attracted a great deal of attention as a vector for gene therapy and is also useful for studying gene functions in differentiated cells such as neurons and muscle cells. An efficient method of constructing recombinant adenoviruses has been established. In this article, we will introduce the method to produce the desired recombinant adenoviruses and show our findings using these adenoviruses.<br>

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