A fundamental study on a bioassay for the antiphlogistic effect of topically applied antiinflammatory agents

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  • YAMADA Katsushi
    Department of Pharmacology, School of Medicine, Fukuoka University
  • HATTORI Shuuzo
    Department of Pharmacology, School of Medicine, Fukuoka University
  • HAKODA Tatsuya
    Department of Pharmacology, School of Medicine, Fukuoka University
  • MIYAZAKI Michiyo
    Department of Pharmacology, School of Medicine, Fukuoka University
  • THO Tamae
    Department of Pharmacology, School of Medicine, Fukuoka University
  • FURUKAWA Tatsuo
    Department of Pharmacology, School of Medicine, Fukuoka University

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  • 抗炎症剤の局所応用による抗浮しゅ効果の検定法
  • コウ エンショウザイ ノ キョクショ オウヨウ ニヨル コウ フシュ コウカ

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Abstract

To establish a reliable laboratory assay for quantitating topical anti-inflammatory efficacy, the method of Tonelli et al was modified by employing felt fixed forceps, felt and sharp punch in Wistar rats. Croton oil, applied topically to the rat ear, elicited an acute phlogistic response which was maximal 6 hr after the application. The phlogistic response elicited by a single topical application of croton oil (1, 2, 5 and 10%) was increased in a dose-dependent manner, and croton oil, 5%, induced 63.2% increase of ear weight and was the optimal concentration for the experiment. Using this procedure, the antiphlogistic potencies of two corticoids were assayed under conditions of a blind test. ED50 of betamethasone valerate and diflucortolone valerate was 0.26 mg/ml and 0.0097 mg/ml, respectively, in Wistar rats, and 0.86 mg/ml and 0.016 mg/ml in Sprague Dawley rats, suggesting that diflucortolone valerate has an antiphlogistic potency of 27-56 relative to that seen with betamethasone valerate with minor differences in the strain of the rat. Our method should prove to be a useful assay for rapidly quantitating antiphlogistic activities of topically applied corticoids.

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