Separation of Cytokinin-like Substances from the Root of Tea and the Changes in the Content during Sprouting of Tea Shoots

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  • チャの根におけるサイトカイニン活性の変動とその分離
  • チャ ノ ネ ニ オケル サイトカイニン カッセイ ノ ヘンドウ ト ソノ ブ

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Abstract

The concentration of cytokinin in the roots increased before sprouting of new shoots, and gradually decreased after sprouting. The amount of cytokinin in the roots reached to the highest value at the just sprouting stage.<BR>It was clarified by sephadex LH 20 column chromatography and polyvinyl-pyrolidone column chromatography that the fraction A extracted by n-butanol from basic aqueous soluiton of the root homogenate had at least three kinds of cytokinin-like substances. Rfs of the main cyto-kinin-like substances found in the root extract on paper chromatogram and thin-layer chromatogram were same as those of zeatin. The UV spectrum of the purified substance had a maximun absorption at 268 nm and coincided with that of zeatin.

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