Examination on Determining Physiological Activities of Tea Roots by TTC Reduction and α-Naphthylamine Oxidation and Its Application

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  • TTCおよびα-ナフチルアミンによる茶樹根の活力測定法ならびにその応用

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There are a considerable number of literatures on the growth of tea roots, but little work on the estimation of their physiological activity has been reported. The enzymatic activity of tea roots, therefore, was evaluated by determining the oxidizing and reducing-power of tissue with a-naphthyl-amine (a-NA) or TTC (2, 3, 5-triphenyl tetrazolium chloride).<BR>Some experimental results on the roots of flooded tea plants were also obtained by using this method.<BR>A highly significant correlation (r=+0.899) between the TTC reductive activity and the a-NA oxidative one was found when the fleshy roots were used. However, the former was far more striking in its reaction than the latter. The TTC reduction method can be recommended for the determination of the physiological activity of tea roots.<BR>Since the root systems of tea plants consist of a large number of roots of various developmental stages like other woody plants, a careful attention should be paid to obtain uniform samples of rootlets in the examination of their physiological activity. The TTC reducing-power of fleshy roots of a tea variety varied slightly with its plant age and growing place.<BR>The physiological activity of tea roots grown under flooding conditions decreased significantly with the increase of flooding days.<BR>It is concluded that the above-mentioned method may be an excellent means to estimate roughly the activity of tea roots grown under various conditions.

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