Abnormal Expression of 8-Nitroguanine in the Brain of Mice Exposed to Arsenic Subchronically
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- PIAO Fengyuan
- Department of Occupational and Environmental Health, Dalian Medical University
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- LI Sheng
- Department of Biochemistry, Dalian Medical University
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- LI Qiujuan
- Department of Occupational and Environmental Health, Dalian Medical University
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- YE Jianxin
- Department of Occupational and Environmental Health, Dalian Medical University
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- LIU Shuang
- Department of Occupational and Environmental Health, Dalian Medical University
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To provide molecular toxicological evidences for exploring the mechanism of arsenic-induced neurotoxicity the accumulation of arsenic (As), the formation of 8-nitroguanine (8-NO2-G) were examined in brain tissue of mice exposed to arsenic. And the gene expressions of inducible NOS (iNOS), superoxide dismutase 1 (SOD1) and peroxiredoxin 2 (Prdx2) were also analyzed by GeneChip. In the result, the concentration of As in the brain tissue of mice was 4.00, 13.70, 21.48 and 29.88 ng/g in the controls and experimental groups exposed to 1, 2 and 4 mg/l As2O3, respectively and increased in dose-response manner. Nervous cells in the brain of mice exposed to As showed disappearances of axons, vacuolar degeneration in cytoplasm and karyolysis, whereas no such pathological changes were observed in the control group. Weak immunoreactivity against 8-NO2-G was observed in the brain tissue of mice given 1 or 2 ppm arsenic trioxide. More intensive immunoreactivity was found in cells at 4 ppm and it was mainly distributed in cytoplasm. The expressions of SOD1 and Prdx2 were down-regulated in the brain of mice exposed to As, but iNOS expression was not disturbed by As exposure. No the 8-NO2-G immunoreactivity or abnormal expressions of these genes in brain tissue were observed in controls. These results indicate that As induces high expression of 8-NO2-G in brain tissues of mice and that RNA in the cells may be modified by overproduced reactive nitrogen species.<br>
収録刊行物
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- Industrial health
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Industrial health 49 (2), 151-157, 2011
独立行政法人 労働者健康安全機構 労働安全衛生総合研究所
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詳細情報 詳細情報について
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- CRID
- 1390001204280726144
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- NII論文ID
- 130004828602
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- NII書誌ID
- AA00672955
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- ISSN
- 18808026
- 00198366
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- NDL書誌ID
- 11059732
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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