Investigation of the Callus Induction Medium and the Regeneration Medium in Rice Anther Culture.

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  • イネやく培養におけるカルス誘導培地と再分化培地の検討
  • イネ葯培養におけるカルス誘導培地と再分化培地の検討
  • イネヤクバイヨウ ニ オケル カルス ユウドウバイチ ト サイブンカバイチ ノ ケントウ

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We determined the callus induction medium and the regeneration medium to efficiently utilize the anther culture technique in rice breeding. The medium containing N6 basal medium supplemented with 4 mg L-1 of 2, 4-D effectively induced calli from microspores of some japonica varieties and two F1 hybrids with japonica varieties. The regenerated plants containing green and/or albino shoots were produced efficiently from microspore-derived calli on the regeneration medium containing LS basal medium supplemented with sorbitol (30000 mg L-1), CH (2000 mg L-1), MES (1000 mg L-1), NAA (2 mg L-1), and kinetin (0.5 mg L-1) solidified by gellun gum (4000 mg L-1).

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