Protective Effect of Histidine on Hydroxyl Radical Generatio Induced by Potassium-Depolarization in Rat Myocardium.
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- Obata Toshio
- Department of Pharmacology, Oita Medical University
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- Aomine Masahiro
- Division of Nutritional Physiology, Graduate School of Nutrition Science, Nakamura Gakuen University
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- Yamanaka Yasumitsu
- Department of Pharmacology, Oita Medical University
Bibliographic Information
- Other Title
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- Protective Effect of Histidine on Hydroxyl Radical Generation Induced by Potassium-Depolarization in Rat Myocardium
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Abstract
We investigated the efficacy of histidine on potassium-depolarization induced hydroxyl radical (·OH) generation in the extracellular fluid of rat myocardium by a flexibly mounted microdialysis technique (O system). After the rat was anesthetized, a microdialysis probe was implanted in the left ventricular myocardium, and then sodium salicylate in Ringer’s solution (0.5 nmol/μl per minute) was infused to detect the generation of ·OH as reflected by the nonenzymatic formation of 2, 3-dihydroxybenzoic acid (DHBA). Infusion of KCl (70 mM) clearly produced an increase in ·OH formation. However, when KCl in the presence of a high concentration of histidine (25 mM) was infused through the microdialysis probe, KCl failed to increase the 2, 3-DHBA formation. To examine the effect of histidine on ischemia-reperfusion of the myocardium, the heart was subjected to myocardial ischemia for 15 min by occlusion of the left anterior descending coronary artery (LAD). When the heart was reperfused, a marked elevation of the levels of 2, 3-DHBA was observed in the heart dialysate. However, when corresponding experiments were performed with histidine (25 mM)-pretreated animals, histidine prevented the ischemia-reperfusion induced ·OH formation trapped as 2, 3-DHBA. These results indicate that histidine may protect against K+-depolarization-evoked ·OH generation in rat myocardium.
Journal
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- The Japanese Journal of Pharmacology
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The Japanese Journal of Pharmacology 80 (3), 217-222, 1999
The Japanese Pharmacological Society
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Details 詳細情報について
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- CRID
- 1390001204284973568
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- NII Article ID
- 10008195231
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- NII Book ID
- AA00691188
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- COI
- 1:CAS:528:DyaK1MXkslKrt7c%3D
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- ISSN
- 13473506
- 00215198
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- NDL BIB ID
- 4806269
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- PubMed
- 10461766
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- Web Site
- http://id.ndl.go.jp/bib/4806269
- https://ndlsearch.ndl.go.jp/books/R000000004-I4806269
- https://api.elsevier.com/content/article/PII:S0021519819308455?httpAccept=text/xml
- https://api.elsevier.com/content/article/PII:S0021519819308455?httpAccept=text/plain
- https://www.jstage.jst.go.jp/article/jjp/80/3/80_3_217/_pdf
- https://search.jamas.or.jp/link/ui/2000024587
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed