(+)-(3H)Isradipine and (3H)Glyburide Bindings to Heart and Lung Membranes From Rats With Monocrotaline-Induced Pulmonary Hypertension.
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- Nakayama Koichi
- Department of Pharmacology, Faculty of Pharmaceutical Sciences, University of Shizuoka
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- Fukuta Yoshihisa
- Department of Pharmacology, Faculty of Pharmaceutical Sciences, University of Shizuoka
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- Kiyoshi Akihiko
- Department of Pharmacology, Faculty of Pharmaceutical Sciences, University of Shizuoka
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- Iwatsuki Yoshiyuki
- Department of Pharmacology, Faculty of Pharmaceutical Sciences, University of Shizuoka
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- Ishii Kunio
- Department of Pharmacology, Faculty of Pharmaceutical Sciences, University of Shizuoka
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- Ishikawa Tomohisa
- Department of Pharmacology, Faculty of Pharmaceutical Sciences, University of Shizuoka
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- Iida Mari
- Biosafety Research Center, Foods, Drugs and Pesticides
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- Iwata Hijiri
- Biosafety Research Center, Foods, Drugs and Pesticides
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- Enomoto Makoto
- Biosafety Research Center, Foods, Drugs and Pesticides
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Abstract
We examined the binding of a 1, 4-dihydropyridine-sensitive Ca2+ channel ligand, (+)-[3H]isradipine (PN200-110), and that of an ATP-sensitive K+ (KATP) channel ligand, [3H]glyburide, to heart, lung and brain membranes isolated from Sprague-Dawley rats made pulmonary hypertensive by monocrotaline, a pyrrolizidine alkaloid. A single subcutaneous injection of monocrotaline increased right ventricular systolic pressure, a measure of pulmonary arterial pressure, and the thickness of the right ventricular free wall in 3 to 4 weeks. The (+)-[3H]PN200-110 and [3H]glyburide binding site densities (Bmax) were reduced in hypertrophied right ventricles when normalized per unit protein in comparison with those of age-matched control (sham) rats, whereas the values of the dissociation constant (Kd) of both ligands bound to the hypertrophied right ventricle were not significantly changed. The [3H]PN200-110 binding to the lung membranes of the monocrotaline-induced pulmonary hypertensive rats was increased. The results indicate that the change in the binding of 1, 4-dihydropyridine Ca2+ and KATP channel ligands to heart membranes may contribute to the pathological alteration of cardiopulmonary structure and functions in rats with pulmonary hypertension induced by monocrotaline.
Journal
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- The Japanese Journal of Pharmacology
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The Japanese Journal of Pharmacology 81 (2), 176-184, 1999
The Japanese Pharmacological Society
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Details 詳細情報について
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- CRID
- 1390001204285682304
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- NII Article ID
- 10008196807
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- NII Book ID
- AA00691188
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- COI
- 1:CAS:528:DyaK1MXmvFahtLg%3D
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- ISSN
- 13473506
- 00215198
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- NDL BIB ID
- 4886658
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- PubMed
- 10591475
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed