Existence of Ionotropic Glutamate Receptor Subtypes in Cultured Rat Retinal Ganglion Cells Obtained by the Magnetic Cell Sorter Method and Inhibitory Effects of 20-Hydroxyecdysone, a Neurosteroid, on the Glutamate Response.
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- Mukai Satoshi
- Department of Ophthalmology, Hiroshima University School of Medicine
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- Mishima Hiromu K.
- Department of Ophthalmology, Hiroshima University School of Medicine
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- Shoge Keisuke
- Department of Ophthalmology, Hiroshima University School of Medicine
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- Shinya Makoto
- Department of Ophthalmology, Hiroshima University School of Medicine
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- Ishihara Kumatoshi
- Department of Pharmacotherapy, Graduate School of Medical Sciences, Hiroshima University
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- Sasa Masashi
- Department of Pharmacology, Hiroshima University School of Medicine
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Abstract
Glutamate and neurosteroids are known to exist in retinal ganglion cells (RGC). Therefore, patch clamp studies using the whole-cell recording method were performed to determine whether or not ionotropic glutamate receptor subtypes, i.e., N-methyl-<sc>D</sc>-aspartate (NMDA), α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and kainate receptors, were present on RGC obtained by the magnetic cell sorter (MACS) method and cultures. In addition, the effects of 20-hydroxyecdysone (20-HE), a neurosteroid, on inward currents induced by NMDA, AMPA and kainate were examined at a holding potential of −60 mV. The current-voltage relationship for NMDA in the presence of glycine and Mg2+-free, as well as those for AMPA and kainate were linear, with a reversal potential of around 0 mV. NMDA-induced currents were blocked by MK-801, while both AMPA- and kainate-induced currents were blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). Application of 20-HE in the bath resulted in significant inhibitions on NMDA-, AMPA- and kainate-induced currents. Thus, NMDA, AMPA and kainate receptors were confirmed to exist on MACS-separated cultured RGC. Moreover, 20-HE inhibited NMDA receptor-mediated currents most prominently and AMPA- and kainate-mediated currents moderately, suggesting that neurosteroids may be playing a role in modulating glutamate-mediated transmission in RGC, and 20-HE might be useful for preventing glutamate neurotoxicity.
Journal
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- The Japanese Journal of Pharmacology
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The Japanese Journal of Pharmacology 89 (1), 44-52, 2002
The Japanese Pharmacological Society
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Keywords
Details 詳細情報について
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- CRID
- 1390001204285988608
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- NII Article ID
- 130000078224
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- NII Book ID
- AA00691188
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- COI
- 1:STN:280:DC%2BD38zktFSmtQ%3D%3D
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- ISSN
- 13473506
- 00215198
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- NDL BIB ID
- 6169375
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- PubMed
- 12083742
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- Web Site
- http://id.ndl.go.jp/bib/6169375
- https://ndlsearch.ndl.go.jp/books/R000000004-I6169375
- https://api.elsevier.com/content/article/PII:S0021519819301465?httpAccept=text/xml
- https://api.elsevier.com/content/article/PII:S0021519819301465?httpAccept=text/plain
- https://search.jamas.or.jp/link/ui/2002259357
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed