Calcium Oscillations in Single Cultured Chinese Hamster Ovary Cells Stably Transfected with a Cloned Human Cholecystokinin (CCK)B Receptor.
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- Akagi Keiko
- Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
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- Nagao Taku
- Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
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- Urushidani Tetsuro
- Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
書誌事項
- タイトル別名
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- Calcium Oscillations in Single Cultured
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In Chinese hamster ovary cells stably expressing the cloned human cholecystokinin (CCK)B /gastrin receptor, cholecystokinin octapeptide (CCK-8) evoked increases in [Ca2+]i monitored by digitized video imaging of fura-2 fluorescence ratios. At concentrations around 10 pM, CCK-8 elicited [Ca2+]i oscillations, which were blocked by elimination of extracellular Ca2+, by a phospholipase C inhibitor, U-73122, by a protein kinase C inhibitor, H7, as well as by phospholipase A2 (PLA2) inhibitors, ONO-RS-082 and aristolochic acid. At higher concentrations, CCK-8 induced a single biphasic [Ca2+]i rise consisting of a large peak followed by a lower sustained plateau, while the response turned into [Ca2+]i oscillation when the extracellular Ca2+ was eliminated or a PLA2 inhibitor was included. CCK-8 stimulated the release of arachidonic acid, and this was inhibited by aristolochic acid. Arachidonic acid caused an increase in [Ca2+]i which was dependent upon extracellular Ca2+. These results suggest that the activation of PLA2 might be involved, at least in part, in the Ca2+ influx that maintains the sustained plateau phase of [Ca2+]i as well as the [Ca2+]i oscillation when CCKB receptors are stimulated.
収録刊行物
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- Jpn.J.Pharmacol.
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Jpn.J.Pharmacol. 75 (1), 33-42, 1997
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390001204287475072
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- NII論文ID
- 10008679679
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- NII書誌ID
- AA00691188
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- COI
- 1:CAS:528:DyaK2sXmtl2qtLw%3D
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- ISSN
- 13473506
- 00215198
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- NDL書誌ID
- 4307643
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- PubMed
- 9334884
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可