Studies on the subsynaptosomal distribution of psychotropic drugs in rat cerebral cortex.

DOI Web Site Web Site Web Site PubMed 被引用文献1件 参考文献15件
  • ISHITANI Ryoichi
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Josai University
  • IWAMOTO Takio
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Josai University

書誌事項

タイトル別名
  • Studies on the subsynaptosomal distribu

この論文をさがす

抄録

After administration of H3-imipramine, H3-dimetacrine and S35-chlorpromazine by the direct lateral intraventricular injection, synaptosomes-rich fraction (F-B) was isolated from rat cerebral cortex by differential and 3-stepwise density gradient centrifugation. The isolated F-B fraction was treated by hyposmotic-lysis followed by freezing and thawing once or 15 times and, furthermore, fractionated into the subsynaptosomal fractions by 5-stepwise density gradient centrifugation. The subsynaptosomal distribution of these drugs showed the same distribution patterns, i.e., the larger portion of radioactivity was recovered in the synaptic ghost membranes-rich fractions. On the other hand, synaptic vesicles-rich fractions contained less radioactivity. On the disrupting process of F-B fraction, when the F-B fraction was treated by hyposmotic-lysis followed by freezing and thawing 15 times, only pellet (FD-6) fraction was obtained as compared with freezing and thawing once (4 interphase layers were obtained). Morphological examination revealed that the synaptic ghost membranes were located in the FD-6 fraction, but morphological damages were not observed. Under these conditions, H3-imipramine showed a 74.1% of release from the synaptosomes, while release with H3-dimetacrine and S35-chlorpromazine was 3.9 and 11.3% respectively.

収録刊行物

  • Jpn.J.Pharmacol.

    Jpn.J.Pharmacol. 27 (6), 755-762, 1977

    公益社団法人 日本薬理学会

被引用文献 (1)*注記

もっと見る

参考文献 (15)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ