A Method for Culturing Human Hair Follicle Cells (II)

DOI 5 Citations 10 References

Bibliographic Information

Other Title
  • Hair Follicle Cellsの培養法(II)
  • A method for culturing human hair follicle cells. (II). Plucked human hair follicles.
  • —Plucked Human Hair Follicles—
  • —抜去毛より採取した毛包の培養法—

Description

We performed an explant culture and a dispersed cell culture of plucked hair follicles and could obtain hair follicle cell colonies without contamination of fibroblasts by both culture methods. To determine the optimal culture condition, MMC treated 3T3 cells, collagen type I membrane (undcr surface: 3T3 cells without MMC treatment) and collagen type IV were used as feeder layers or substrate. The hair follicle cell growth could be constantly viewed only on the collagen type IV coated dishes. Therefore, it is suggested that collagen type IV, a component of the basement membrane, is essential for the growth of hair follicle cells from plucked human hair follicles.

Journal

  • Nishi Nihon Hifuka

    Nishi Nihon Hifuka 50 (2), 271-276, 1988

    Western Division of Japanese Dermatological Association

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References(10)*help

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Keywords

Details 詳細情報について

  • CRID
    1390001204298860032
  • NII Article ID
    130004473343
  • DOI
    10.2336/nishinihonhifu.50.271
  • ISSN
    18804047
    03869784
    http://id.crossref.org/issn/03869784
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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