A Method for Culturing Human Hair Follicle Cells (I)

DOI 2 Citations 6 References

Bibliographic Information

Other Title
  • Hair Follicle Cellsの培養法(I)
  • A method for culturing human hair follicle cells. (I). A method for culturing hair follicles obtained from surgically resected scalp.
  • —A Method for Culturing Hair Follicles Obtained from Surgically Resected Scalp—
  • —手術摘出頭皮より採取した毛包の培養法—

Abstract

We cultured human hair follicle cells from follicles of the surgically resected scalp skin. In the presence of calcium (1.96 mM), a physiological concentration, the cells piled up, keratinized and rapidly displayed a terminal differentiation, as seen in skin epidermal cells. Only when they were cultured from the start with a physiological calcium concentration medium and then with a low calcium concentration one (0.43 mM) did monolayered, polygonal and large nucleated basal cell-like cells appear, and were retained for about 20 days. To remove the fibroblasts which grow rapidly and surround hair follicle cell colonies in the course of culture, we scraped them off with a rubber policeman and the growth was suppressed to some extent.

Journal

  • Nishi Nihon Hifuka

    Nishi Nihon Hifuka 50 (1), 97-102, 1988

    Western Division of Japanese Dermatological Association

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References(6)*help

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Keywords

Details 詳細情報について

  • CRID
    1390001204298994304
  • NII Article ID
    130004695198
  • DOI
    10.2336/nishinihonhifu.50.97
  • ISSN
    18804047
    03869784
    http://id.crossref.org/issn/03869784
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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