Non-destructive DNA extraction protocol for oribatid mites (Acari: Oribatida)

DOI JASI Web Site 23 References
  • Ota Aino
    Graduate School of Environment and Information Sciences, Yokohama National University
  • Karasawa Shigenori
    Fukuoka University of Education
  • Nakamura Tatsuo
    Graduate School of Environment and Information Sciences, Yokohama National University
  • Harada Hiroshi
    Graduate School of Environment and Information Sciences, Yokohama National University
  • Shimano Satoshi
    Environmental Education Center, Miyagi University of Education

Bibliographic Information

Other Title
  • ササラダニ類に関する非破壊的DNA抽出法の検討

Search this article

Abstract

The molecular analysis of oribatid mites for identification and phylogeny is usually conducted through PCR amplification and DNA sequencing, with DNA extracted from a ground and completely destroyed specimen. To overcome the difficulty of associating molecular and morphological information, we propose a non-destructive DNA extraction protocol using enzymatic lysis of proteinase K during 48hr incubation on single mite individuals. The procedure has been tested on three rather different species-Atropacarus striculus (C.L. Koch, 1835) (Phthiracaridae), Heminothrus (Platynothrus) peltifer (C.L. Koch, 1839) (Camisiidae), and Protoribates sp. AO-2010 (Protoribatidae)-targeting the sequence of the D3 region of the 28S rRNA gene with preservation of the exoskeleton for further study. This procedure enables systematic analysis with morphological information by linking the morphology and genotype from a single mite.

Journal

  • Edaphologia

    Edaphologia 89 (0), 19-24, 2011

    The Japanese Society of Soil Zoology

References(23)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top