Impact of Biomimetic Diamond-like Carbon coated Titanium on Osteoblast and Osteoclast Differentiation in vitro

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  • Mine Yuichi
    Department of Oral Biology & Engineering, Integrated Health Sciences, Institute of Biomedical and Health Sciences, Hiroshima University
  • Nakatani Tatsuyuki
    Toyo Advanced Technologies Co., Ltd.
  • Okamoto Keishi
    Toyo Advanced Technologies Co., Ltd.
  • Hara Shintarou
    Toyo Advanced Technologies Co., Ltd.
  • Takagi Kentaro
    Department of Oral Biology & Engineering, Integrated Health Sciences, Institute of Biomedical and Health Sciences, Hiroshima University
  • Nikawa Hiroki
    Department of Oral Biology & Engineering, Integrated Health Sciences, Institute of Biomedical and Health Sciences, Hiroshima University

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To examine the effects of hydrogen amount-altered biomimetic DLC film, osteoblastic MC3T3-E1 cells and osteoclastic RAW264.7 cells were cultured on titanium (Ti) disks coated with or without DLC including various amount of hydrogen. The amount of hydrogen in DLC film is reduced from 20.9% to 0.8%. Real-time quantitative reverse transcriptase-polymerase chain reaction analysis showed that typeⅠcollagen mRNA in MC3T3-E1 cells cultured on specific hydrogen-containing DLC coated Ti disks was significantly higher than those of control Ti disks. In addition, specific hydrogen-containing DLC coated Ti disks significantly inhibited the expression of TRAP mRNA in RANKL-treated RAW264.7 cells. These results, taken together, suggested that biomimetic DLC altered osteoblast and osteoclast differentiation, which could promise long-term success of implant therapy.

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