Isolation of novel gain- and loss-of-function alleles of the circadian clock gene LATE ELONGATED HYPOCOTYL (LHY) in Arabidopsis
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- Oda Atsushi
- Institute of Biological Sciences, University of Tsukuba Max Planck Institute for Plant Breeding
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- Reeves Paul H
- Max Planck Institute for Plant Breeding
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- Tajima Takeomi
- Institute of Biological Sciences, University of Tsukuba
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- Nakagawa Mayu
- Institute of Biological Sciences, University of Tsukuba
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- Kamada Hiroshi
- Institute of Biological Sciences, University of Tsukuba
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- Coupland George
- Max Planck Institute for Plant Breeding
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- Mizoguchi Tsuyoshi
- Institute of Biological Sciences, University of Tsukuba
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Description
In Arabidopsis, two myb-related proteins, LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), play key roles in the control of circadian rhythms. Photoperiodic flowering and elongation of hypocotyls are regulated by a circadian clock. Here, we report the isolation and characterization of a novel deletion allele (lhy-14) and a gain-of-function allele (lhy-2) of lhy. Based on long hypocotyl and late flowering phenotypes under long days, lhy-2 was isolated as an intragenic suppressor of lhy-12, one of the loss-of-function alleles of lhy. Although the lhy-12 mRNA has a 19-bp truncation, we found that this was not caused by a deletion in the LHY but by a point mutation. We propose a model explaining the loss-of-function of LHY in lhy-12 and the partial suppression of the lhy-12 phenotype by lhy-2, by the abnormal splicing of LHY mRNA.
Journal
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- Plant Biotechnology
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Plant Biotechnology 24 (5), 457-465, 2007
Japanese Society for Plant Biotechnology
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Keywords
Details 詳細情報について
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- CRID
- 1390001204326642688
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- NII Article ID
- 10021912118
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- NII Book ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 9302411
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed