Generation of pink flower varieties from blue Torenia hybrida by redirecting the flavonoid biosynthetic pathway from delphinidin to pelargonidin
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- Nakamura Noriko
- Institute for Plant Science, Suntory Holdings Ltd.
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- Fukuchi-Mizutani Masako
- Institute for Plant Science, Suntory Holdings Ltd.
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- Fukui Yuko
- Institute for Plant Science, Suntory Holdings Ltd.
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- Ishiguro Kanako
- Institute for Plant Science, Suntory Holdings Ltd.
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- Suzuki Kenichi
- Suntory Flowers, Ltd.
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- Suzuki Hideyuki
- Kazusa DNA Research Institute
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- Okazaki Koei
- Kazusa DNA Research Institute
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- Shibata Daisuke
- Kazusa DNA Research Institute
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- Tanaka Yoshikazu
- Institute for Plant Science, Suntory Holdings Ltd.
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Transgenic torenia plants with various pink petal tones were obtained from blue or violet cultivars by combining downregulation of endogenous flavonoid 3′-hydroxylase (F3′H) and flavonoid 3′, 5′-hydroxylase (F3′5′H) genes with expression of a heterologous gene. Knockdown of the F3′H and F3′5′H genes in a blue torenia cultivar resulted in pale pink lines by decreasing the cyanidin and delphinidin levels and increasing the pelargonidin-based anthocyanin levels. Additional expression of rose dihydroflavonol 4-reductase (DFR) gene elevated the level of pelargonidin and yielded darker pink petals. Expression of pelargonidin DFR gene instead of the rose DFR gene increased the level of pelargonidin and darkened the petal color. Introducing the two genetic constructs containing a DFR gene into a violet torenia cultivar, which had more anthocyanins and a darker color than the blue one, further increased the level of pelargonidin and pink color intensity. These results reveal that selection of a suitable gene source and host greatly affects the phenotypes of the resultant transgenic plants.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 27 (5), 375-383, 2010
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390001204326844928
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- NII論文ID
- 10027702291
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- NII書誌ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 10926348
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 使用不可