Induction of Somatic Embryogenesis in Carrot by Osmotic Stress.
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- KAMADA Hiroshi
- Gene Exteriment Center, University of Tsukuba
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- ISHIKAWA Keiko
- Faculty of Horticulture, Chiba University
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- SAGA Hitoshi
- Gene Exteriment Center, University of Tsukuba
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- HARADA Hiroshi
- Gene Exteriment Center, University of Tsukuba
Bibliographic Information
- Other Title
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- 浸透圧ストレスによるニンジン不定胚形成の誘導
- 浸透圧ストレスによるニンジン不定胚形成の誘導〔英文〕
- シントウアツ ストレス ニ ヨル ニンジン フテイ ハイ ケイセイ ノ ユウド
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Abstract
Segments of cotyledon and apical tip taken from 1-week-old seedlings of Daucus carota L. were cultured for 3 weeks on hormone-free Murashige and Skoog's (MS) medium containing high amounts of sucrose (0.3-0.7M) and then transferred to the same medium with lower sucrose concentration (0.09M). Somatic embryos were formed from the segments 3 to 6 weeks after the transfer. On the other hand, hypocotyl segments given the same treatment did not produce somatic embryos. When the primary culture medium contained 0.61M mannitol and 0.09M sucrose, cotyledon segments and apical tip segments also produced somatic embryos when transferred to hormone-free MS medium with 0.09M sucrose. In all cases, somatic embryos were formed directly from the explants without visible callus formation.
Journal
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- Plant tissue culture letters
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Plant tissue culture letters 10 (1), 38-44, 1993
Japanese Society for Plant Cell and Molecular Biology
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Details 詳細情報について
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- CRID
- 1390001204326890240
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- NII Article ID
- 130001066171
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- NII Book ID
- AN10050931
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- ISSN
- 18847706
- 02895773
- http://id.crossref.org/issn/0229009X
- http://id.crossref.org/issn/08454213
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- NDL BIB ID
- 3828839
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed