Identification of the <i>glutathione</i> S-<i>transferase</i> gene responsible for flower color intensity in carnations

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  • Sasaki Nobuhiro
    Department of Biotechnology, Tokyo University of Agriculture and Technology
  • Nishizaki Yuzo
    Department of Biotechnology, Tokyo University of Agriculture and Technology
  • Uchida Yasuhiro
    Department of Biotechnology, Tokyo University of Agriculture and Technology
  • Wakamatsu Eigo
    Department of Biotechnology, Tokyo University of Agriculture and Technology
  • Umemoto Naoyuki
    Central Laboratories for Frontier Technology, Kirin Holdings Co., Ltd.
  • Momose Masaki
    Central Laboratories for Frontier Technology, Kirin Holdings Co., Ltd.
  • Okamura Masachika
    Central Laboratories for Frontier Technology, Kirin Holdings Co., Ltd.
  • Yoshida Hiroyuki
    Applied Plant Research Laboratory, Japan Tobacco, Inc.
  • Yamaguchi Masaatsu
    Faculty of Enviromental Horticulture, Minami Kyushu University
  • Nakayama Masayoshi
    Institute of Floricultural Science, National Agriculture and Food Research Organization
  • Ozeki Yoshihiro
    Department of Biotechnology, Tokyo University of Agriculture and Technology
  • Itoh Yoshio
    Department of Biotechnology, Tokyo University of Agriculture and Technology

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  • Identification of the glutathione S-transferase gene responsible for flower color intensity in carnations

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Abstract

Two cDNAs with homology to glutathione S-transferase (GST) were isolated from the carnation (Dianthus caryophyllus); these cDNAs are termed here DcGSTF1 and DcGSTF2. Phylogenetic analysis suggested that both DcGSTF1 and DcGSTF2 belonged to the Phi class of GSTs. DcGSTF2 showed high levels of transcription at late stages of petal development when anthocyanin biosynthesis is most active. Sequencing of DcGSTF2 indicated that it consisted of three exons and two introns. A truncated DcGSTF2 gene, resulting from the insertion of a CACTA-type transposable element, was found in the genome of a mutable flower line bearing deep pink sectors on pale pink petals. A full length DcGSTF2 gene driven by a continuous expression promoter was introduced into the epidermal cells of carnations with pale pink petals. The transformed cells were deep pink. These results suggest that the DcGSTF2 gene is responsible for flower color intensity in carnations.

Journal

  • Plant Biotechnology

    Plant Biotechnology 29 (3), 223-227, 2012

    Japanese Society for Plant Biotechnology

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