Gateway vectors for plant transformation

DOI Web Site 19 Citations 38 References
  • Nakagawa Tsuyoshi
    Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
  • Ishiguro Sumie
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • Kimura Tetsuya
    Department of Sustainable Resource Science, Graduate School of of Bioresources, Mie University

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Abstract

The availability of complete plant genome sequences has opened a new era of plant molecular research using approaches such as comprehensive expression analysis, ectopic expression and gene knockdown. For such purposes, new tools realizing easy and rapid construction of recombinant plasmids are required. Here, we describe Gateway cloning compatible binary vectors for post-genomic research in plant genetic engineering. The vectors comprise a variety of reporters, epitope tags and selective markers that should make them useful for construction of plasmids for Agrobacterium-mediated transformation of plants. We also describe vectors for promoter swapping using Gateway cloning technology.

Journal

  • Plant Biotechnology

    Plant Biotechnology 26 (3), 275-284, 2009

    Japanese Society for Plant Biotechnology

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