Gateway vectors for plant transformation
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- Nakagawa Tsuyoshi
- Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
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- Ishiguro Sumie
- Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
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- Kimura Tetsuya
- Department of Sustainable Resource Science, Graduate School of of Bioresources, Mie University
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Abstract
The availability of complete plant genome sequences has opened a new era of plant molecular research using approaches such as comprehensive expression analysis, ectopic expression and gene knockdown. For such purposes, new tools realizing easy and rapid construction of recombinant plasmids are required. Here, we describe Gateway cloning compatible binary vectors for post-genomic research in plant genetic engineering. The vectors comprise a variety of reporters, epitope tags and selective markers that should make them useful for construction of plasmids for Agrobacterium-mediated transformation of plants. We also describe vectors for promoter swapping using Gateway cloning technology.
Journal
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- Plant Biotechnology
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Plant Biotechnology 26 (3), 275-284, 2009
Japanese Society for Plant Biotechnology
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Keywords
Details 詳細情報について
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- CRID
- 1390001204326915456
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- NII Article ID
- 10024853760
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- NII Book ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 10258681
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed