Mapping Major Replication Origins on the Rice Plastid DNA.

DOI Web Site 参考文献53件
  • WANG Ying
    United Graduate School of Agricultural Sciences, Iwate University
  • TAMURA Kohya
    Cryobiosystem Research Center, Iwate University
  • SAITOH Yasushi
    United Graduate School of Agricultural Sciences, Iwate University Cryobiosystem Research Center, Iwate University
  • SATO Tadashi
    Department of Ecology and Evolutionary Biology, Graduate School of Life Science, Tohoku University
  • HIDAKA Soh
    Department of Crop Breeding, National Agricultural Research Center for Tohoku Region
  • TSUTSUMI Ken-ichi
    United Graduate School of Agricultural Sciences, Iwate University Cryobiosystem Research Center, Iwate University

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To maintain and to differentiate into various plastid lineages, replication of the plastid DNA (ptDNA) and division of the plastid must take place. However, replication initiation of the ptDNA has been less understood. The present study describes identification of the initiation region (origin) of ptDNA replication in the rice cultured cells. RNA-primed newly replicated DNA strands pulse-labeled with bromodeoxyuridine were isolated and size-fractionated. Locations of these nascent strands on the ptDNA determined the two major origin regions around the 3' region of each 23S rDNA in the inverted repeats (IRA and IRB). Two-dimensional agarose gel electrophoresis of the replication intermediates suggested that replication from each origin proceeds bidirectionally. This contrasted to replication by the double D-loop mechanism.

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