Ｔｒｉｃｈｏｓｐｏｒｏｎ　ｐｅｎｉｃｉｌｌａｔｕｍのペクチナーゼによる植物葉肉プロトプラストの単離

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書誌事項

タイトル別名
  • Isolation of plant mesophyll protoplasts with an endo-polygalacturonase from Trichosporon penicillatum.
  • Trichosporon penicillatum ノ ペクチナーゼ ニ ヨル

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Plant mesophyll protoplasts with a endo-polygalacturonase produced by Trichosporon penicillatum SNO-3 was isolated. The following mesophyll tissues of shoot were used for the isolation: Tagetes minuta, Brassica rapa, Raphanus sativus, Lactuca sativa, Triticum aestivum, Secale cereale, Hordeum vulgare, Panicum crusgalli, Avena sativa, Zeamays and Oryza sativa. Pectinase SE-150, a partially purified endo-polygalacturonase, combined with cellulase ONOZUKA RS liberated protoplasts from all mesophyll tissues tested.<br>The protoplast yields were 1-4×106 per gram fresh weight. Cell division and colony formation from lettuce protoplasts obtained with a pectinase SE-150 enzyme system were also observed. The percentage of colony formation on the 14th day after incubation in a culturing medium was estimated to be approximately 18%.

収録刊行物

  • 植物組織培養

    植物組織培養 7 (1), 14-18, 1990

    日本植物細胞分子生物学会

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