RNAi suppression of the anthocyanidin synthase gene in Torenia hybridia yields white flowers with higher frequency and better stability than antisense and sense suppression
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- Nakamura Noriko
- Institute for Advanced Technology, Suntory, Ltd.
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- Fukuchi-Mizutani Masako
- Institute for Advanced Technology, Suntory, Ltd.
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- Miyazaki Kiyoshi
- Institute for Advanced Technology, Suntory, Ltd.
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- Suzuki Kenichi
- Suntory Flowers, Ltd.
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- Tanaka Yoshikazu
- Institute for Advanced Technology, Suntory, Ltd.
書誌事項
- タイトル別名
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- RNAi suppression of the anthocyanidin synthase gene in Torenia hybrida yields white flowers with higher frequency and better stability than antisense and sense suppression
この論文をさがす
抄録
Post-transcriptional gene silencing, such as antisense suppression, sense suppression (or cosuppression), and RNAi, is often used to down-regulate a target gene in transgenic plants. Novel flower color is industrially important; furthermore, flower color is a convenient tool to monitor the stability of such silencing. Previously, we obtained white torenia through sense suppression of chalcone synthase or dihydroflavonol 4-reductase (Suzuki et al. 2000). However, their phenotypes were not stable. In this study, we suppressed the anthocyanidin synthase gene using three methods in transgenic torenia. About half of the transgenic torenia plants gave white flowers by RNAi suppression of the gene, while antisense and sense suppression yielded a few and no white flowers, respectively. The white flower color obtained by RNAi has been stable for three years in a greenhouse. This study shows the usefulness of RNAi to suppress a target gene.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 23 (1), 13-17, 2006
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390001204327249152
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- NII論文ID
- 10021907687
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD28XktFaqsrg%3D
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 7857071
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可