Investigating the role of vitamin C in tomato through TILLING identification of ascorbate-deficient tomato mutants

DOI Web Site 3 Citations 51 References
  • Baldet Pierre
    INRA Bordeaux, UMR 1332 Biologie du Fruit et Pathologie University of Bordeaux, UMR 1332
  • Bres Cécile
    INRA Bordeaux, UMR 1332 Biologie du Fruit et Pathologie University of Bordeaux, UMR 1332
  • Okabe Yoshihiro
    Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Mauxion Jean-Philippe
    INRA Bordeaux, UMR 1332 Biologie du Fruit et Pathologie University of Bordeaux, UMR 1332
  • Just Daniel
    INRA Bordeaux, UMR 1332 Biologie du Fruit et Pathologie University of Bordeaux, UMR 1332
  • Bournonville Céline
    INRA Bordeaux, UMR 1332 Biologie du Fruit et Pathologie University of Bordeaux, UMR 1332
  • Ferrand Carine
    INRA Bordeaux, UMR 1332 Biologie du Fruit et Pathologie University of Bordeaux, UMR 1332
  • Mori Kentaro
    INRA Bordeaux, UMR 1332 Biologie du Fruit et Pathologie University of Bordeaux, UMR 1332 Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Ezura Hiroshi
    Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Rothan Christophe
    INRA Bordeaux, UMR 1332 Biologie du Fruit et Pathologie University of Bordeaux, UMR 1332

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Abstract

Besides being a model for fleshy fruits and Solanaceae species, tomato represents one of the main sources of ascorbate (vitamin C) in human diet in many parts of the world. Ascorbate fulfills various roles in plants due to its antioxidant potential and to its connection with other metabolic pathways e.g. cell wall biosynthesis. Among the functional genomic tools recently developed in tomato, EMS (ethyl methanesulfonate) mutant collections provide an opportunity for identifying allelic series of mutations in target genes by TILLING (Targeting Induced Local Lesions IN Genomes). We describe here the use of tomato EMS mutant collections in the miniature cv. Micro-Tom for the discovery of allelic variants in three ascorbate biosynthetic genes encoding the GDP-D-mannose pyrophosphorylase (GMP), the GDP-D-mannose epimerase (GME) and the GDP-L-galactose phosphorylase (GGP) respectively. We report on the discovery of several missense, truncation and splice junction mutations in these genes affecting plant ascorbate content to various levels, and show that several tomato mutant lines with strongly reduced ascorbate content undergo severe bleaching upon exposure to high light intensity.

Journal

  • Plant Biotechnology

    Plant Biotechnology 30 (3), 309-314, 2013

    Japanese Society for Plant Biotechnology

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