Simple cryopreservation protocol with an encapsulation technique for tobacco BY-2 suspension cell cultures
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- Kobayashi Toshihiro
- Experimental Plant Division, BioResource Center, RIKEN Tsukuba Institute
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- Niino Takao
- Genebank, National Institute of Agrobiological Sciences
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- Kobayashi Masatomo
- Experimental Plant Division, BioResource Center, RIKEN Tsukuba Institute
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Abstract
Tobacco BY-2 cells were successfully cryopreserved by a simple slow prefreezing (equilibrium freezing) method using an encapsulation technique. After the cells were immobilized in alginate gel beads, the beads were treated with cryoprotectant solution (2 M glycerol, 0.4 M sucrose) for 45 min. The beads were then transferred to a laboratory freezer at −30°C, stored for 2 h, and then immersed in liquid nitrogen. To initiate the regrowth of cells, the beads were warmed in a water bath. Following dilution of cryoprotectant solution, the beads were suspended and cultured in normal medium. With this method, suspension cell cultures were regrown within 7 days. There were no differences in the morphology or growth profiles between cryopreserved cell cultures and the original cell cultures.
Journal
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- Plant Biotechnology
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Plant Biotechnology 22 (2), 105-112, 2005
Japanese Society for Plant Biotechnology
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Details 詳細情報について
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- CRID
- 1390001204327393664
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- NII Article ID
- 10026526727
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- NII Book ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 7374280
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed