Molecular Identification of Two Genes of Germin-like Protein in Arabidopsis.

  • SAGE-ONO Kimiyo
    Institute of Biological Sciences, University of Tsukuba
  • ONO Michiyuki
    Biotechnology Institute, Aktia Prefectural College of Agriculture
  • OGUCHI Taichi
    Institute of Biological Sciences, University of Tsukuba
  • HASEBE Masako
    Biotechnology Institute, Aktia Prefectural College of Agriculture
  • XU Zeng-Jun
    Biotechnology Institute, Aktia Prefectural College of Agriculture
  • UEDA Kenji
    Biotechnology Institute, Aktia Prefectural College of Agriculture
  • INOUE Masayasu
    Biotechnology Institute, Aktia Prefectural College of Agriculture
  • KAMADA Hiroshi
    Institute of Biological Sciences, University of Tsukuba

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  • Molecular Identification of Two Genes o

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Description

We identified two homolog genes of PnGLP (Pharbitis nil germin-like protein) and SaGLP (Sinapis alba GLP) in Arabidpsis thaliana, namely, AtGLP1 and AtGLP2. Results of Southern blotting showed that each gene was single per haploid genome. Northern blotting revealed the presence of mRNAs for both genes in all overground parts, but especially in the leaf and flower. RFLP mapping showed that AtGLP1 was on chromosome 1 (112.4±0.6cM) and AtGLP2 was on chromosome 5 (35.7±0.3cM).

Journal

  • Plant Biotechnology

    Plant Biotechnology 15 (2), 103-108, 1998

    Japanese Society for Plant Biotechnology

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