Highly efficient system for plant regeneration from leaf and stem explants in Dahlia

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  • Highly efficient system for plant regeneration from leaf and stem explants in <i>Dahlia</i>

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Protocol of plant regeneration from leaf and stem explants was established in dahlia (Dahlia×pinnata) ‘Yamatohime’ in in vitro culture. Nodular calli were successfully induced from leaf and stem explants on 30 g l−1 sucrose-containing MS medium supplemented singly with one of the cytokinins, 6-benzyladenine (BA), zeatin or thidiazuron (TDZ) and the highest frequency of nodular callus induction was obtained on medium containing 10 mg l−1 TDZ. The nodular callus induced on this medium subsequently produced a tissue with numerous shoot primordial structures called ‘mass of shoot primordia’ (MSP), which was maintained at least for more than 2 years on the same medium. Shoot induction occurred after transfer the MSP onto hormone-free MS medium, in which replacement of sucrose by maltose was necessary. The shoots excised from the MSP were rooted on hormone-free MS medium and successfully grown under greenhouse conditions. The regenerated plants showed the same morphological characteristics to those of the control plants and flowered normally.

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