Microscale alkaline nitrobenzene oxidation method for high-throughput determination of lignin aromatic components
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- Yamamura Masaomi
- Research Institute for Sustainable Humanosphere, Kyoto University
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- Hattori Takefumi
- Research Institute for Sustainable Humanosphere, Kyoto University
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- Suzuki Shiro
- Institute of Sustainability Science, Kyoto University
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- Shibata Daisuke
- Kazusa DNA Research Institute
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- Umezawa Toshiaki
- Research Institute for Sustainable Humanosphere, Kyoto University Institute of Sustainability Science, Kyoto University
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説明
The nitrobenzene oxidation method is widely used for structural analysis of lignin. However, the conventional nitrobenzene oxidation method has several drawbacks including the requirement of a sizeable amount of sample material and the rather slow completion of the reaction process. In this paper, we describe a microscale nitrobenzene oxidation method using deuterium-labeled vanillin and syringaldehyde as internal standards. Using this method, we show that the microscale nitrobenzene oxidation method realized high-throughput determination of lignin components using a small amount of sample, i.e. 10 mg per reaction, and high reproducibility. Due to the small sample sizes, the oxidation reaction and extraction steps of a number of samples can be completed in parallel, thus enabling us to process more than 40 samples per day.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 27 (4), 305-310, 2010
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390001204327677696
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- NII論文ID
- 10026705904
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BC3cXhsVegsLzK
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 10833001
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可