Production of transgenic Itarian ryegrass(Lolium multiflorum Lam.) via microprojectile bombardment of embryogenic callus
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- TAKAHASHI Wataru
- Japan Grassland Farming Forage Seed Association, Forage Crop Research Institute
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- OISHI Hideki
- Japan Grassland Farming Forage Seed Association, Forage Crop Research Institute
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- EBINA Masumi
- National Institute of Livestock and Grassland Science
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- TAKAMIZO Tadashi
- National Institute of Livestock and Grassland Science
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- KOMATSU Toshinori
- National Institute of Livestock and Grassland Science
書誌事項
- タイトル別名
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- Production of Transgenic Italian Ryegrass (Lolium multiflorum Lam.) via Microprojectile Bombardment of Embryogenic Calli.
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説明
We established a microprojectile-bombardment-mediated transformation system in embryogenic calli of Italian ryegrass (Lolium multiflorum Lam.). Bombardment conditions with a PDS-1000/He helium-driven biolistic device were optimized by transient expression assays of a chimeric β-glucuronidase (GUS) gene construct. The highest number of transient GUS expression events was observed with 24 h of high osmotic pretreatment and a bombardment pressure of 7584 kPa. Diploid and tetraploid Italian ryegrass calli were transformed under optimal conditions with two plasmids harboring the gene for antisense expression of cinnamyl alcohol dehydrogenase (CAD) or hygromycin phosphotransferase (HPT). Transformed calli were obtained via microprojectile bombardment followed by selection of transformants on media containing hygromycin at 50mg l-1 for 1 week and then 100mg l-1 for 5 weeks. A total of 33 transgenic plants were produced. The transformation frequencies in cultivars Waseaoba (diploid) and Meritra (tetraploid) were 3.8% and 2.3%, respectively. The total cointegration frequencies of the HPT and antiCAD genes (antisense orientation of CAD gene) were 55.6% in Waseaoba and 73.3% in Meritra. The expression of the antiCAD gene in the co transformed plants was confirmed by RT-PCR.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 19 (4), 241-249, 2002
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390001204327812736
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- NII論文ID
- 10010149303
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD38Xpslaqsrk%3D
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- ISSN
- 13476114
- 13424580
- http://id.crossref.org/issn/13424580
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- NDL書誌ID
- 6373694
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可