Gene note: An acyltransferase-like gene obtained by differential gene expression profiles of quinolizidine alkaloid-producing and nonproducing cultivars of Lupinus angustifolius
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- Bunsupa Somnuk
- Graduate School of Pharmaceutical Sciences, Chiba University
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- Okada Taketo
- Graduate School of Pharmaceutical Sciences, Chiba University
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- Saito Kazuki
- Graduate School of Pharmaceutical Sciences, Chiba University RIKEN Plant Science Center
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- Yamazaki Mami
- Graduate School of Pharmaceutical Sciences, Chiba University CREST, Japan Science and Technology Agency (JST)
書誌事項
- タイトル別名
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- An acyltransferase-like gene obtained by differential gene expression profiles of quinolizidine alkaloid-producing and nonproducing cultivars of Lupinus angustifolius
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抄録
Quinolizidine alkaloids (QAs) are one of the representative groups of plant alkaloids. To isolate genes involved in QA biosynthesis, we performed a differential gene expression analysis by PCR-select subtraction between a QA-producing bitter cultivar and a nonproducing sweet cultivar of Lupinus angustifolius. We obtained 71 and 43 clones specific to the bitter and sweet cultivars, respectively. Among the genes specifically expressed in the bitter cultivar, an acyltransferase-like gene (LaAT: Lupinus angustifolius acyltransferase) showing homology to the BAHD protein family was isolated. LaAT showed the strongest homology to the Arabidopsis thaliana BAHD acyltransferases involved in the formation of conjugated polyamines. Semi-quantitative RT-PCR revealed that LaAT expression was highest in the young leaves but barely detectable in the other organs of the bitter cultivar plant, whereas LaAT expression was undetectable in the sweet cultivar.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 28 (1), 89-94, 2011
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390001204327932544
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- NII論文ID
- 10028258703
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- NII書誌ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 11021388
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可