Starvation Treatment Increases Transient Expression Frequency of a Foreign Gene Introduced into Eustoma grandiflorum Root Tissues by Particle Bombardment.

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  • TAKAHASHI Misa
    Graduate Department of Gene Science, Faculty of Science, Hiroshima University
  • IRIFUNE Kohei
    Graduate Department of Gene Science, Faculty of Science, Hiroshima University
  • HASEGAWA Masanori
    Graduate Department of Gene Science, Faculty of Science, Hiroshima University
  • KURODA Hiroyuki
    Wood Research Institute, Kyoto University
  • NISHIZAWA Shuji
    Bio-Technology group, Basic Research Laboratories and Nagano Prefecture Agriculture Corporation Federation
  • MORIKAWA Hiromichi
    Graduate Department of Gene Science, Faculty of Science, Hiroshima University

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  • 飢餓処理がパーティクルガンによりトルコギキョウ根組織に導入されたトランスジーンの一過的遺伝子発現効率を上昇させる
  • Starvation Treatment Increases Transien

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Root explants of Eustoma grandiflorum were bombarded with plasmid pBI221 harboring the uidA gene encoding β-glucuronidase (GUS) driven by a cauliflower mosaic virus 35S promoter, and the effects of pre- and post-bombardment culture in various starvation media on the number of blue spots of GUS-expressing cells were studied. It was found that pre- and post-bombardment culture in nitrogen-depleted media increased the expression frequency of the transgene, by 4- to 8-times that of the control, and that 3-h pre-culture in the nitrogen-depleted medium gave the highest expression frequency.

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