Agrobacterium-mediated transformation of the wild orchid species Phalaenopsis amabilis

DOI Web Site 被引用文献1件 参考文献59件
  • Semiarti Endang
    Faculty of Biology, Gadjah Mada University Center Study of Biotechnology, Gadjah Mada University
  • Indrianto Ari
    Faculty of Biology, Gadjah Mada University
  • Purwantoro Azis
    Faculty of Agriculture, Gadjah Mada University
  • Isminingsih Sulastri
    Center Study of Biotechnology, Gadjah Mada University
  • Suseno Nilo
    Faculty of Biology, Gadjah Mada University
  • Ishikawa Takaaki
    Division of Biological Science, Graduate School of Science, Nagoya University Plant Biology Research Center, Chubu University
  • Yoshioka Yasushi
    Division of Biological Science, Graduate School of Science, Nagoya University
  • Machida Yasunori
    Division of Biological Science, Graduate School of Science, Nagoya University
  • Machida Chiyoko
    Plant Biology Research Center, Chubu University College of Bioscience and Biotechnology, Chubu University

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Phalaenopsis hybrids constitute a major ornamental crop. An important parent species for many of these hybrids is Phalaenopsis amabilis. We developed a convenient method for the genetic modification of P. amabilis using Agrobacterium tumefaciens. The transformed intact protocorms, which are young orchid seedlings of P. amabilis, regenerated plants under the same conditions that showed the highest frequency of shooting. A kanamycin resistance gene under the control of the 35S promoter can be used as a selective marker. In addition, T-DNA vectors containing the Arabidopsis class 1 KNOX gene, BP/KNAT1, were successfully introduced into protocorms. Shoots were generated with an abnormal leaf shape that was easily distinguished from that of normal shoots, indicating that BP/KNAT1 can be used as a visible marker gene. Furthermore, the protocorms transformed with BP/KNAT1 produced multiple shoots. Both the presence and expression of the transgene in transformed plants were confirmed by molecular analysis.

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