Agrobacterium-mediated transformation of the wild orchid species Phalaenopsis amabilis
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- Semiarti Endang
- Faculty of Biology, Gadjah Mada University Center Study of Biotechnology, Gadjah Mada University
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- Indrianto Ari
- Faculty of Biology, Gadjah Mada University
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- Purwantoro Azis
- Faculty of Agriculture, Gadjah Mada University
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- Isminingsih Sulastri
- Center Study of Biotechnology, Gadjah Mada University
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- Suseno Nilo
- Faculty of Biology, Gadjah Mada University
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- Ishikawa Takaaki
- Division of Biological Science, Graduate School of Science, Nagoya University Plant Biology Research Center, Chubu University
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- Yoshioka Yasushi
- Division of Biological Science, Graduate School of Science, Nagoya University
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- Machida Yasunori
- Division of Biological Science, Graduate School of Science, Nagoya University
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- Machida Chiyoko
- Plant Biology Research Center, Chubu University College of Bioscience and Biotechnology, Chubu University
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Description
Phalaenopsis hybrids constitute a major ornamental crop. An important parent species for many of these hybrids is Phalaenopsis amabilis. We developed a convenient method for the genetic modification of P. amabilis using Agrobacterium tumefaciens. The transformed intact protocorms, which are young orchid seedlings of P. amabilis, regenerated plants under the same conditions that showed the highest frequency of shooting. A kanamycin resistance gene under the control of the 35S promoter can be used as a selective marker. In addition, T-DNA vectors containing the Arabidopsis class 1 KNOX gene, BP/KNAT1, were successfully introduced into protocorms. Shoots were generated with an abnormal leaf shape that was easily distinguished from that of normal shoots, indicating that BP/KNAT1 can be used as a visible marker gene. Furthermore, the protocorms transformed with BP/KNAT1 produced multiple shoots. Both the presence and expression of the transgene in transformed plants were confirmed by molecular analysis.
Journal
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- Plant Biotechnology
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Plant Biotechnology 24 (3), 265-272, 2007
Japanese Society for Plant Biotechnology
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Keywords
Details 詳細情報について
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- CRID
- 1390001204328347392
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- NII Article ID
- 10021911313
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- NII Book ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD2sXosVejs7o%3D
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 8775114
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed