Isolation and Functional Analysis of the Promoter Sequence of the Cry j 1 Gene, Which Encodes a Major Allergenic Protein in the Pollen of Japanese Cedar (Cryptomeria japonica)

DOI Web Site 1 Citations 42 References Open Access
  • YAMADA Shinya
    Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology
  • KAJITA Shinya
    Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology
  • SHIOKAWA Takahiro
    Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology
  • MOROHOSHI Noriyuki
    Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology

Bibliographic Information

Other Title
  • Isolation and functional analysis of the promoter sequence of the Cry j 1 gene, which encodes a major allergenic protein in the pollen of Japanese ceder (Cryptomeria japonica)

Search this article

Description

We isolated promoter sequences of the Cry j 1 gene-which encodes an allergenic protein in the pollen of Japanese cedar-by an inverse-PCR technique using genomic DNA as the template. One of the isolated promoter sequences conferred pollen-associated expression on a fusion construct that included a gene for β-glucuronidase (GUS) in transgenic tobacco plants. Histochemical analysis of GUS expression using four deletion mutants of this promoter sequence revealed that expression differed among pollen grains at different stages of maturation and that a 223-bp 5’-untranslated region of the gene was sufficient for spatial and temporal expression of the gene in pollen and pollen tubes. Our results indicate that the promoter sequence might be adequate for active pollen-associated expression in tobacco, as it is in Cryptomeria japonica.

Journal

  • Plant Biotechnology

    Plant Biotechnology 20 (3), 241-245, 2003

    Japanese Society for Plant Biotechnology

Citations (1)*help

See more

References(42)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top