Functional genomics of the Dof transcription factor family genes in suspension-cultured cells of Arabidopsis thaliana
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- Tsujimoto-Inui Yayoi
- Institute for Biological Resources and Functions, AIST
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- Naito Yuki
- Institute for Biological Resources and Functions, AIST
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- Sakurai Nozomu
- Kazusa DNA Research Institute
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- Suzuki Hideyuki
- Kazusa DNA Research Institute
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- Sasaki Ryosuke
- Kazusa DNA Research Institute
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- Takahashi Hideyuki
- Institute of Molecular and Cellular Biosciences (IMCB), The University of Tokyo
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- Ohtsuki Namie
- Institute for Biological Resources and Functions, AIST
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- Nakano Toshitsugu
- Institute for Biological Resources and Functions, AIST
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- Yanagisawa Shuichi
- Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)
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- Shibata Daisuke
- Kazusa DNA Research Institute
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- Uchimiya Hirofumi
- Institute of Molecular and Cellular Biosciences (IMCB), The University of Tokyo
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- Shinshi Hideaki
- Institute for Biological Resources and Functions, AIST
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- Suzuki Kaoru
- Institute for Biological Resources and Functions, AIST
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Abstract
The Dof (DNA-binding with one finger) genes are members of a family of plant-specific transcription factors that have a highly conserved DNA-binding domain, namely, Dof domain. The Dof domain is a particular class of zinc finger domain that has been demonstrated to bind specifically to DNA sequences with a T/AAAAG core. In the Arabidopsis genomic database, 36 Dof genes have been identified, whereas the functions of most of the members still remain to be studied. Therefore, we attempted to comprehensively and systematically investigate functions of Arabidopsis Dof genes. As the first step, we isolated cDNAs of all the Arabidopsis Dof genes based on the coding sequences identified on the genomic database. Then, we selected genes, which are subjected to further functional analysis, through a phylogenetic analysis, and transformed Arabidopsis cultured cells (line T87) using cDNAs corresponding to the selected genes. After that, we examined transcriptional profiles in the Dof gene-overexpressed calli using an Arabidopsis DNA microarray. From the results, possible involvements of the Dof genes in regulation of metabolic pathways are discussed.
Journal
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- Plant Biotechnology
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Plant Biotechnology 26 (1), 15-28, 2009
Japanese Society for Plant Biotechnology
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Keywords
Details 詳細情報について
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- CRID
- 1390001204328786816
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- NII Article ID
- 10025180958
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- NII Book ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 10196831
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed