Ectopic expression of an oat thionin gene in carnation plants confers enhanced resistance to bacterial wilt desease

DOI Web Site 参考文献28件
  • SHIRASAWA-SEO Naomi
    Miyagi Prefectural Agriculture and Horticulture Research Center
  • NAKAMURA Shigeo
    Miyagi Prefectural Agriculture and Horticulture Research Center
  • UKAI Naomi
    Miyagi Prefectural Agriculture and Horticulture Research Center
  • HONKURA Ryoso
    Miyagi Prefectural Agriculture and Horticulture Research Center
  • IWAI Takayoshi
    Miyagi Prefectural Agriculture and Horticulture Research Center National Institute of Agrobiological Sciences
  • OHASHI Yuko
    National Institute of Agrobiological Sciences Core Research for Evolutional Science and Technology

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  • Ectopic Expression of an Oat Thionin Gene in Carnation Plants Confers Enhanced Resistance to Bacterial Wilt Disease.

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Under the control of Arabidopsis triptophan synthase β subunit promoter (PTSB1), the oat thionin gene (Asthi1) was introduced into carnation (Dianthus caryphyllus L.) via Agrobacterium-mediated gene transfer. Leaf bases of three cultivars of carnation (‘Scania’, ‘Percian Pink-Sim’ and ‘U. Conn. White Sim’) were infected with Agrobacterium harboring the binary vector with PTSB1::Asthi1 and hygromycin genes, and cultured on the medium containing hygromycin. Independent 99 lines resistant to hygromycin were regenerated and the introduction of the transgene was confirmed in 11 lines by polymerase chain reaction. Transgenic carnation plants exhibited the resistance against infection with Burkholderia caryophylli which caused bacterial wilt, and the levels of resistance correlated with those of the thionin gene transcript.

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