MYB-mediated upregulation of lignin biosynthesis in Oryza sativa towards biomass refinery
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- Koshiba Taichi
- Research Institute for Sustainable Humanosphere, Kyoto University
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- Yamamoto Naoki
- Research Institute for Sustainable Humanosphere, Kyoto University
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- Tobimatsu Yuki
- Research Institute for Sustainable Humanosphere, Kyoto University
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- Yamamura Masaomi
- Research Institute for Sustainable Humanosphere, Kyoto University
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- Suzuki Shiro
- Research Institute for Sustainable Humanosphere, Kyoto University
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- Hattori Takefumi
- Research Institute for Sustainable Humanosphere, Kyoto University
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- Mukai Mai
- Research Institute for Sustainable Humanosphere, Kyoto University
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- Noda Soichiro
- Research Institute for Sustainable Humanosphere, Kyoto University
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- Shibata Daisuke
- Kazusa DNA Research Institute
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- Sakamoto Masahiro
- Graduate School of Agriculture, Kyoto University
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- Umezawa Toshiaki
- Research Institute for Sustainable Humanosphere, Kyoto University Research Unit for Global Sustainability Studies, Kyoto University
書誌事項
- タイトル別名
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- MYB-mediated upregulation of lignin biosynthesis in <i>Oryza sativa</i> towards biomass refinery
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説明
Lignin encrusts lignocellulose polysaccharides, and has long been considered an obstacle for the efficient use of polysaccharides during processes such as pulping and bioethanol fermentation. However, lignin is also a potential feedstock for aromatic products and is an important by-product of polysaccharide utilization. Therefore, producing biomass plant species exhibiting enhanced lignin production is an important breeding objective. Herein, we describe the development of transgenic rice plants with increased lignin content. Five Arabidopsis thaliana (Arabidopsis) and one Oryza sativa (rice) MYB transcription factor genes that were implicated to be involved in lignin biosynthesis were transformed into rice (O. sativa L. ssp. japonica cv. Nipponbare). Among them, three Arabidopsis MYBs (AtMYB55, AtMYB61, and AtMYB63) in transgenic rice T1 lines resulted in culms with lignin content about 1.5-fold higher than that of control plants. Furthermore, lignin structures in AtMYB61-overexpressing rice plants were investigated by wet-chemistry and two-dimensional nuclear magnetic resonance spectroscopy approaches. Our data suggested that heterologous expression of AtMYB61 in rice increased lignin content mainly by enriching syringyl units as well as p-coumarate and tricin moieties in the lignin polymers. We contemplate that this strategy is also applicable to lignin upregulation in large-sized grass biomass plants, such as Sorghum, switchgrass, Miscanthus and Erianthus.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 34 (1), 7-15, 2017
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390001204328877568
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- NII論文ID
- 130005519594
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- NII書誌ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 028091792
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 使用不可