Plant regeneration from Crotalaria spectabilis hairy roots which showed inhibited growth of root-knot nematodes (Meloidogyne hapla and M. incognita) in vitro

DOI Web Site 参考文献23件
  • Ohara Akiko
    Graduate School of Horticulture, Chiba University
  • Daimon Hiroyuki
    Graduate School of Life and Environmental Sciences, Osaka Prefecture University
  • Momota Yoji
    NARO Institute of Crop Science, National Agriculture and Food Research Organization
  • Chin Dong Poh
    Graduate School of Horticulture, Chiba University
  • Mii Masahiro
    Graduate School of Horticulture, Chiba University

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  • Plant regeneration from <i>Crotalaria spectabilis</i> hairy roots which showed inhibited growth of root-knot nematodes (<i>Meloidogyne hapla</i> and <i>M. incognita</i>) <i>in vitro</i>

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Hairy roots were induced from leaf segments of Crotalaria spectabilis, which is used as a green manure crop antagonistic to nematodes, by infection with a mikimopine type wild strain of Agrobacterium rhizogenes A13 (MAFF02-10266). For initial proliferation of hairy roots after induction, addition of gibberellin A3 to the medium was indispensable. These roots exhibited vigorous growth and abundant lateral branching on half-strength Murashige and Skoog (1/2MS) medium without plant growth regulators. The adventitious shoots were induced from 24% of root segments 2 months after transfer onto B5 medium containing 5 mg l－1 6-benzyladenine. The axillary buds excised from these shoots produced roots 1 month after transfer onto 0.4% (wv－1) gellan gum-solidified 1/2MS medium containing 30 mM fructose without plant growth regulators. Regenerated plants were successfully grown under greenhouse conditions. Infection of hairy roots of C. spectabilis with root-knot nematodes Meloidogyne hapla and M. incognita led to the growth inhibition of these nematodes.

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