Suitable Conditions for the Induction and Micropropagation of PLBs in Some Monopodial Orchids.
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- KISHI Fumiaki
- Central Laboratories for Key Technology, Kirin Brewery Co., Ltd.
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- KAGAMI Yuukichi
- Plant Laboratory, Kirin Brewery Co., Ltd.
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- TAKAGI Koujirou
- Takagi Orchid Nursery Co.
Bibliographic Information
- Other Title
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- Suitable Conditions for the Induction a
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Abstract
Liquid media suitable for the induction and micropropagation of protocorm-like bodies (PLBs) in Vandofinetia Nara ‘Yumika Pink’ were developed by modifying MS basal medium. The best medium obtained for PLBs induction was one-fourth strength MS medium containing 30g/l sucrose and for PLBs micropropagation was one-fourth strength MS medium containing 10g/l sucrose in which FeEDTA was replaced by 6.95mg/l FeSO47H2O. Usefulness of the latter medium was confirmed in other monopodial orchids such as Renanetia, Darwinara, Ascocentrum and Ascofinetia. After transfer onto 2g/l Hyponex (N:P:K=10:30:20) medium containing 2g/l proteose peptone and 20g/l sucrose for 2 months, the PLBs (both those regenerating and those not regenerating plantlets) were subcultured into MS medium containing 30g/l sucrose at 2 months intervals for 4 months. More than 10, 000 plantlets ready for transplanting to pots were obtained efficiently from one floral bud within a year for each orchid cultivar. These results suggest that the methodology could be used on a commercial scale for micropropagation of a wide range of monopodial orchids.
Journal
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- Plant Biotechnology
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Plant Biotechnology 14 (1), 17-21, 1997
Japanese Society for Plant Biotechnology
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Details 詳細情報について
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- CRID
- 1390001204329565824
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- NII Article ID
- 10009276041
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- NII Book ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 4252185
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed