Micropropagation of Fritillaria camtschatcensis (L.) Ker-Gawl., "Kuroyuri"

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  • OTANI Motoyasu
    Laboratory of Plant Cell Breeding, Research Institute of Agricultural Resources, Ishikawa Agricultural College,
  • SHIMADA Takiko
    Laboratory of Plant Cell Breeding, Research Institute of Agricultural Resources, Ishikawa Agricultural College,

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  • 白山のクロユリ,Firitillaria camtschatcensis (L.) Ker-Gawl.,の組織培養による増殖
  • Micropropagation of Firitillaria camtschatcensis(L.)Ker-Gawl.,"Kuroyuri"
  • Micropropagation of Firitillaria camtsc

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Abstract

Fritillaria camtschatcensis (L.) Ker-Gawl., "Kuroyuri" is a native perennial plant in Mt. Hakusan. To establish a method for in vitro propagation of Kuroyuri explants of bulbscales and leaves were cultured on the media containing plant growth regulators (hormones). NAA and picloram promoted the adventitious bulblet formation on the explants; almost all explants of bulbscales produced an average of five adventitious bulblets each on the LS medium (containing 3% of sucrose) supplemented with 0.1 mg L^<-1> of NAA or 0.1 mg L^<-1> of picloram. After transferring onto the medium without plant hormones adventitious bulblets grew to generate leaves and roots. A high concentration of sucrose (6% and 9%) had no stimulatory effects on the bulblet growth.

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