PCR法を用いて検査した牛体外作出脱出胚盤胞の性比
書誌事項
- タイトル別名
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- The Sex Ratio of Bovine Hatched Blastocysts Produced and Cultured In Vitro as Determined by the Polymerase Chain Reaction
抄録
The sex ratio of bovine hatched blastocysts produced and cultured in vitro was examined by the polymerase chain reaction (PCR). A total of 190 hatched blastocysts was obtained between 188 and 248 h post insemination (hpi). The hatching rates expressed as a percentage of the total one-cell presumptive zygotes (0 hpi) and of cleaved embryos (72 hpi) were 31.8 and 46.7%, respectively. The sex of 189 embryos (99.5%) was successfully determined. The results of agarose gel electrophoresis clearly indicated that male embryos gave a double band with male-specific and gender-neutral primers, and females gave a single band with gender-neutral primers alone. The overall sex ratio was 51.9%(98/189), which did not significantly differ from the expected ratio of 1:1. Based on the observations at 12 h intervals, embryos were divided into three developmental groups according to the timing of hatching. The sex ratio of the fast group (188-200 hpi) was shifted to males (59.4%, 38/64), although with no significantly difference from the expected ratio of 1:1. On the other hand, the intermediate (212 hpi) and slow (224-248 hpi) groups had slightly more females than males (47.1%, 33/70) and the sex ratio close to the expected ratio of 1:1 (49.1%, 27/55), respectively.
収録刊行物
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- 哺乳動物卵子学会誌
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哺乳動物卵子学会誌 10 (1), 1-10, 1993
日本哺乳動物卵子学会
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詳細情報 詳細情報について
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- CRID
- 1390001204338621568
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- NII論文ID
- 130004037573
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- ISSN
- 18846513
- 09167625
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可