In vitro exposure of bovine immature oocytes to K99+Escherichia coli and elimination of exposed bacteria by washing
-
- OTOI Takeshige
- Tokushima Prefectural Beef Cattle and Swine Experiment Station
-
- TACHIKAWA Susumu
- Tokushima Prefectural Beef Cattle and Swine Experiment Station
-
- KONDO Shoji
- Tokushima Prefectural Beef Cattle and Swine Experiment Station
-
- SUZUKI Tatsuyuki
- United Graduate School of Veterinary Sciences, Yamaguchi University
Bibliographic Information
- Other Title
-
- 牛未成熟卵子のK99保有大腸菌への曝露と洗浄による細菌除去
Description
Immature bovine oocytes were exposed in vitro to K99+Escherichia coli (K99 E. coli) to determine if K99 E. coli would adhere to the cumulus cells-oocyte complexes or adhere to the cumulus cells-free oocytes. And standard procedures for washing and treating embryos were evaluated to determine their effectiveness for removing K99 E. coli. K99 E. coli was isolated from all groups of cumuluscells-oocyte complexes and 2 of 3 groups of cumulus cells-free oocytes after in vitro exposure and subsequent washing. According to increase in the number of repeated washing, a total number of K99 E. coli isolated from washing medium declined. However after the eighth time of washing, the number of K99 E. coli isolated from washing medium did not decline in the groups of cumulus cells-oocytes complexes. These results indicated that standard procedures for washing were not effective for removing K99 E. coli adhered to immature oocytes and were not able to remove K99 E. coli adhered to the cumulus cells-oocyte complexes.
Journal
-
- Journal of Mammalian Ova Research
-
Journal of Mammalian Ova Research 9 (1), 21-26, 1992
Japanese Society of Mammalian Ova Research
- Tweet
Details 詳細情報について
-
- CRID
- 1390001204338698752
-
- NII Article ID
- 130003839352
-
- ISSN
- 18846513
- 09167625
-
- Data Source
-
- JaLC
- CiNii Articles
-
- Abstract License Flag
- Disallowed