Induction of Differentiation into Endoderm, Insulin-Secreting Cells from Mouse Embryonic Stem Cells Using Activin A and Retinoic Acid
-
- Sato Kahei
- Department of Applied Biological Science, Nihon University College of Bioresource Sciences
-
- Yamamoto Mizuki
- Department of Applied Biological Science, Nihon University College of Bioresource Sciences
この論文をさがす
抄録
Embryonic stem (ES) cells have been known to differentiate into various progenitor cells. In this study, we investigated the differentiation capacity of mouse ES cells into pancreatic hormone-secreting cells, and insulin-secreting cells. ES cells were cultured in Dulbecco’s modified Eagle medium (DMEM) after removal of leukemia inhibitory factor (LIF) for 3 days and then transferred in DMEM supplemented with retinoic acid or activin A. When the culture of embryoid bodies (EBs) derived from ES cells in DMEM added with activin A (2 × 10-9 M or 2 × 10-10 M) for 5 days was performed, endoderm marker genes, GATA4, Sox17 and Foxa2 were expressed in the EBs. However, at 6 days of culture, expression of Sox17 was not observed. When EBs were cultured with activin A (2 × 10-9 M) for 6 days, and followed by 6 days of culture with retinoic acid (10-6 M), expression of the pancreatic cell marker genes, GATA4 and Fxa2, were continued, and pancreatic islet genes, insulins 1 and 2, glucagons and somatostatin, were expressed from 5 days of culture. Immunohistochemistrical analysis gave results similar to RT-PCR. We consider this differentiation method for definitive endoderm production and pancreatic hormone—secreting cells, by using activin A and, retinoic acid is considered to be effective.<br>
収録刊行物
-
- 日本哺乳動物卵子学会誌
-
日本哺乳動物卵子学会誌 29 (1), 22-26, 2012
日本卵子学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001204338766208
-
- NII論文ID
- 130004436826
- 10030590554
-
- NII書誌ID
- AN10548943
-
- ISSN
- 13475878
- 13417738
-
- NDL書誌ID
- 023754018
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可