Phosphatidylinositol 3-Kinase in Cumulus Cells is Responsible for Meiotic Progression from M I to M II Stage in Porcine Follicular Oocytes.
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- Shimada Masayuki
- Faculty of Applied Biological Science, Hiroshima University
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- Anas Mohamed-Kheir Idris
- Graduate School for International Development and Cooperation, Hiroshima University
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- Terada Takato
- Faculty of Applied Biological Science, Hiroshima University
Bibliographic Information
- Other Title
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- ブタ卵胞卵子の第一減数分裂中期(M I)から第二減数分裂中期(M II)への減数分裂進行における卵丘細胞内PI 3-kinaseの役割
- Phosphatidylinositol 3-Kinase in Cumulus Cells is Responsible for Meiotic Progression from M1 to M2 Stage in Porcine Follicular Oocytes
- Phosphatidylinositol 3-Kinase in Cumulu
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Abstract
Porcine cumulus oocyte complexes (COCs) were cultured in an inhibitor free-medium for 24 h and then some of them were denuded. The COCs and denuded oocytes (DOs) were further cultured for 24 h in the presence of Phosphatidylinositol 3-kinase (PI 3-kinase) specific inhibitors, wortmannin and LY294002. In COCs and DOs, wortmannin (10-8, 10-7 M) and LY294002 (5.0 × 10-5 M) significantly increased the proportion of oocytes arrested at M I and decreased that of oocytes reaching M II. Nevertheless, in DOs the differences between in the presence of the inhibitors and the control in the proportion of oocytes reaching M II substantially diminished as compared to those in the case of COCs. Cumulus cell expansion was significantly suppressed by wortmannin and LY294002. It is concluded that PI 3-kinase in cumulus cells plays a regulatory role in meiotic progression beyond M I and cumulus cell expansion in porcine COCs.<br>
Journal
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- Journal of Mammalian Ova Research
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Journal of Mammalian Ova Research 15 (1), 68-76, 1998
JAPANESE SOCIETY OF OVA RESEARCH
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Details 詳細情報について
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- CRID
- 1390001204338815872
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- NII Article ID
- 10013835156
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- NII Book ID
- AN10548943
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- ISSN
- 13475878
- 13417738
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- NDL BIB ID
- 4448318
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed