書誌事項
- タイトル別名
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- Fertilizability of Mouse Eggs Treated with Mannosidase
- マンノシダーゼ ショチ シタ マウス ランシ ノ ジュセイノウ
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抄録
Using zona-free mouse eggs treated with mannosidase, the role of mannose on the surface of eggs in fertilization was examined. The fertilization rates of eggs treated with mannosidase at concentrations of 0.05, 0.1, 0.5 or 1.0 units/100 μl were 47.8, 40.4, 28.2 and 18.8%, respectively, which were all significantly lower than the 69.2% of control eggs with no mannosidase treatment. The binding of Canavalia ensiformis agglutinin (Con A), a lectin with an affinity to mannose, was strong on the surface of all control eggs not treated with mannosidase. However, the rate of eggs showing strong Con A-binding was significantly decreased among those treated with mannosidase at a concentration of 0.1 units/100 μl (44.0%). These findings suggest that mannose on the surface of the cytoplasmic membrane, in addition to zona pellucida, of eggs has a possible role in sperm recognition.<br>
収録刊行物
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- 日本哺乳動物卵子学会誌
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日本哺乳動物卵子学会誌 28 (2), 89-93, 2011
日本卵子学会
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詳細情報 詳細情報について
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- CRID
- 1390001204339260544
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- NII論文ID
- 10028124186
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- NII書誌ID
- AN10548943
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- ISSN
- 13475878
- 13417738
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- HANDLE
- 10191/25134
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- NDL書誌ID
- 11104558
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可